No, I hooked up a keyboard and mouse on day one, and they did not work.
As for Matrix, Gloom, Rogue, or any other SP expansion for Quake 2.
When you assign a key to set the game, you have to press that key once a game is already started, then it will tell you that those options will be active next time you start Quake 2. But when you start a new game, it does infact load the new settings, because I was able to run on walls and stuff because of the Matrix MOD, the only problem is that it simply loads you into the same old Quake 2 levels with the same old weapons, it is as if Quake 2 insists that you set your game to Matrix or what ever, on start up, which is NOT possible without a menu of some sort which is exactly what I have written the author ten million times about, and have revieved no responce. :/
If ANYONE can find a way to get around this problem, please let me know, the death match agianst a PC is kool and all, but what would really get my rocks off is to play all those enhanced single player missions for Quake 2.